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Lesson 3-2 Option 1: Analyzing the Ivory DNA (LAB)

Page history last edited by mariaelizabethbunn@... 8 years, 10 months ago

Lesson 

Time

Engaging the Student (Entry Task) 

Developing the Ideas--Lesson

Checking for Understanding (exit ticket)

Student Handout 
Teacher/Lesson Notes
Materials

90 minutes

Review the scenario addendum from the elicitation lesson, then hand out and review the student lab introduction below: 

View the Full Teacher Guides here:

 

Laboratory Parts:

  • Part 1: Students prepare the gels and DNA samples (this usually takes 30-40 minutes)
  • Part 2: Students run their gels (while gels are running, have students work on their "Concept Questions" handout)
  • Part 3: Students calculate RFLP lengths using banding patterns and size approximation* 

 

*Important Note: Teachers have a choice in how students will further analyze their gel data in Lesson 3-3. You may choose to have students only do the first 7 steps of Part 3 so that the size approximation option can take place in Lesson 3-3 or so that you can instead use the semi-log plot option also outlined in Lesson 3-3. 

 

Laboratory Objectives: 

  1. To understand the physical properties of DNA molecules that allows their separation by agarose gel electrophoresis and apply this technique to an engaging scenario.
  2. To understand the relevance of this technique to scientific research and global issues.
  3. To provide students with further experience with the techniques of electrophoresis, micropipetting, and sample preparation.

 

 

 

 

 

  • Gel Electrophoresis Kit, including freezer box with DNA samples (provided by SEP) 
  • Microwave ovens or hot plates
  • Balances
  • Deionized or distilled water (recommended, but not essential)

Below is a 10-question worksheet that will serve as an exit ticket for this lab. Depending on time, you may wish to pick less questions and create a smaller exit ticket.

 

 

 

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